New technology of three-dimensional multi-layer cultivation of black fungus in hanging bag

First, the season according to the row and the raw material preparation: spring cultivation ear ear row out of the ear two months before the preparation of material, bag; autumn cultivation ear ear row in front of the bag (both high temperature bacteria), after the autumn ( Low-temperature ear production) cultivation; according to the size of cultivation, prepare cotton seed shell, bran, lime and so on. Second, mixed material bagging: Formula 1: 93% cotton shell, bran 5%, lime 2-3%; Formula 2: 45% cotton shell, wood chips 45%, 8% bran, lime 1.5-2% Formulation 3: corncob 30%, cotton seed shell 30%, apple wood chips 20%, bran 18%, gypsum 1%, lime 0.2-1%. Specific approach: the cotton shell skin soaked with water, remove and drench excess water, the lime is evenly sieved into the bran and then well mixed with a good soaked cotton shell, so that the ratio of material to water 1:1.4; After loading, the bag of 15300.04cm high-density polyethylene is required to be packed and evenly distributed. The weight per bag after bagging is about 0.7kg (dry material 0.25kg/bag). Third, sterilization: autoclave 2.5-4 hours. Normal pressure 100 °C, sterilization 16-24 hours. IV. Inoculation: If self-contained strains need to be cultivated 10-15 days before inoculation, the bacteria should be cultured. Using ear-type low-temperature varieties, if sufficient time for bacteria, inoculated with a bag mouth (a little inoculation), such as the time close to the ear, can use Zhadai Zhakou, two points on both sides of the paste sheet vaccination. Such as the purchase of bacteria need to be customized or suitable for use with the purchase of bacteria. A little inoculation, each Bottle of cultivar can be connected to 50-80 bag cultivation bag, two-point inoculation can be connected to 40-45 bag cultivation bag. Fifth, mycelium culture: in advance to do a good job of culturing room finishing sterilization and other work, the training room requirements no light, temperature 20-24 °C, relative humidity of 55-60%, with appropriate ventilation conditions. Sixth, raises the bacteria: In order to increase the output, the good bacteria bag must carry on the bacteria treatment, in order to accumulate the full nutrition source, according to the different species and the season raises the bacteria to be 20-60 days, must keep in the light condition under the culture, and Increase ventilation. VII. Construction of the canopy: Choose a place with ventilation, sunny, good water source, and smooth ground. The canopy floor is 270cm wide, 180cm high, 160cm high, and 5 layers. Each layer is 30cm high. Every two meters can hold 400 bags. The length depends on the number of bags; there should be a film outside the awning when it is used for keeping moisture when the temperature is low, and it can prevent natural rainfall when the temperature is too high; 80-95% of the black mesh is shaded from the top 100cm. Eight, break ear breeding: a good bag, the average temperature in spring rose to above 8 °C, the average temperature in the fall fell below 18 °C, according to 6-8 bags per break "V" type mouth can not be large The total length is not more than 2cm, and the depth is 0.2-0.3cm. When the mouth is well-drawn, the bag will be tied to the frame with the original tie rope. The bag distance is 20cm. The hanging bag in each canopy must be completed within 1-2 days. At the same time, the relative humidity of the air should be increased by 88%-94% for 5-7 days. When the ear base is about to be formed, it will be intermittently sprayed. The relative humidity of the air is always kept at 88-94%, until the ear buds form into the soybean relative humidity by decreasing the size of the beans and enter the management phase of the fruiting body. Nine, fruiting body growth period management: When the ear bud grows to the size of soybeans, the relative humidity of the air is reduced to 83-90%, and the artificial humidification keeps the edge of the ear wet all the time. The relative humidity of the air is kept between 75-94%. It can be used to spray water in the morning and at night and water at noon so that the ears are dry during the day and grow at night, dry and wet until harvested. X. Harvesting and Drying: Early spring, late autumn and autumn cultivation ears can be ear pieces conjoined, a large number of shot spores harvest, late spring, early autumn, summer ear, can be thin in the ear or high temperature ventilation, ears have other When the hyphae emerged, they were immediately harvested and water was stopped 1 day before harvest. Select sunny days to harvest in the morning, harvest, pick large and stay small, try not to destroy the medium; harvest fungus, immediately placed on a bamboo sieve to cool; base down, dried and wrapped in plastic woven bags, coats Transparent bag moisture-proof. 11. Ear management: After the first harvest, the water was stopped for 2-3 days. During the water shut down, each bag was detached from the culture medium after the bag was damaged according to the harvested condition. The “V” mouth is further demarcated; when the outside temperature is high, the 2-3 “V” mouths can be evenly cut into each bag of the Yu’erba ears to increase the density of the ears, spray water, and humidify the first ear. the same. XIII. Pest Control: Black fungus pest control must be “prevented”. In the bag making process, first, there must be sources of pure bacteria without physiological diseases, and then the seed production environment must be strictly treated to prevent environmental cross-infection and the occurrence of fly and fly. Once an infection occurs, it is promptly dumped and the fly is promptly used as a pesticide. Spraying; In the cultivation process, the pollution source nearby must be excluded firstly. During the spraying process, the pollution bag is found to be treated in time. The fly or other flies and flies can be trapped and killed, and pesticides must not be used to avoid pesticide residues.

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