Vitamin A is an oil solution made from 1 g of vitamin A acetate crystals containing more than 2.7 million units of refined vegetable oil. Vitamin A capsules for vitamin A, plus refined edible vegetable oil (de-solid fat at around 0 °C) dissolved and adjusted to make the concentration. The Chinese version of the “2005 Pharmacopoeia†appendix (VII J) contains the determination method for its content.
The method reported in the literature:
Mali et al used RP-HPLC to determine vitamin A content. Instrument: Shimadzu LC-10AT. Column: Huupersil ODS (150 mm 4.6 mm); mobile phase 96% methanol; flow rate: 1.0 ml/min; detection wavelength: 325 nm; column temperature: 25°C.
Huang Shuanglu et al. dissolved the capsules of vitamin A capsules with hydrochloric acid and extracted the contents with cyclohexane. The content was determined by ultraviolet spectrophotometry. The maximum absorption peak wavelength was between 326 and 329 nm.
Li Zhiduan et al. used a three-point calibration method to determine vitamin A content. Vitamin A acetate determination wavelength: λ1 = 316 nm, λ2 = 328 nm, λ3 = 340 nm; vitamin A alcohol determination wavelength: λ1 = 310 nm, λ2 = 325 nm, λ3 = 334 nm.
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