PeproTech cytokines do not contain carrier protein or other additives (such as BSA, HAS or sucrose), and are usually lyophilized with a minimum amount of salt, so trace amounts of cytokines are deposited during lyophilization. Within the tube, a thin or invisible layer of protein is formed. Therefore, we recommend that after receiving the product, it is necessary to centrifuge before the lid is opened, so that the protein stuck on the tube cap or the tube wall gathers at the bottom of the tube (it is normal to see white precipitate at this time).
1. Centrifugation: Centrifugal at high speed (10, 000 rpm) for 20-30 seconds, or low speed centrifugation (2, 000 rpm) for 5 minutes.
2. Reconstitution: Dissolve in deionized water or other buffer (selected according to the instructions) to the concentration required by the instructions (usually 0.1-1.0 mg/mL, such as 10ug product, 10uL-100uL deionized water) Or the buffer is dissolved). Do not oscillate when dissolved, to avoid protein inactivation due to chemical bond cleavage, gently shake with a suitable solvent and let stand for a period of time, after the cytokine is completely dissolved. Solvent selection: Â
1) For products that require deionized water to be dissolved, the salt solution must not be used to avoid irreversible precipitation of proteins due to excessive salt concentration;
2) For products that require dissolution with a salt solution, please follow the concentration on the instruction sheet to avoid excessive salt concentration.
3. Aliquot and Storage: After protein solubilization, it can be further diluted into working fluid according to your own experiment. It can be diluted with RPMI 1640, DMEM or PBS, preferably containing 5-10% FCS (calf or Fetal bovine serum) or 0.5% BSA (bovine serum albumin) to stabilize the protein. The working solution can be stored at 4 ° C for 1 week. If long-term storage is required, it should be stored frozen at -20 ° C (Note: not stored at -80 ° C). The volume of the working fluid in each tube at the time of dispensing is preferably the amount of one experiment, so that one working solution is used in each experiment to avoid the decrease in protein activity caused by repeated freezing and thawing.
Fluorochemicals
The Fluorochemicals compound is a new field in modern organic chemistry. It has a special physical and chemical properties, so it is widely used in the earliest development of applied pesticides and rat poison. During World War II it was developed in some countries appliedas antimicrobial agents, there are more and more new research suggests that fluorochemical will play an increasingly more important role in the field of anticancer drugs. We started later in the field of fluorochemicals, but we have a strong sense of his enormous potential, and we are increasing investment to catch up. We will custom product which has potential market prospects from gram and kilogram-class through process optimization, enlarged to the size of ton. Currently we have developed more fluorochemical compounds in the benzoic acid range, the number of products have been more than 2017.
Organic Fluorochemical Compound,FluorineCompounds,Containing Benzoic Acids
Taizhou Volsen Chemical Co., Ltd. , https://www.volsenchem.com