Abstract, through minded, Yin, blood Therapeutic four different medicine, research mice improved memory models obstacle by Yixin gas, more effective methods of treating memory disorders compared screening TCM. The acquired memory impairment model was treated with the scopolamine hydrobromide method . The behavioral test was performed by the platform method and the dark-avoiding method . At the same time, the normal, model, and western medicine ( nimodipine ) groups were established for comparison. Results STM measurement, model group platform and avoidance reactions error times compared with the normal group significantly increased in each treatment group five minutes the number of errors compared with the model group was significantly decreased, jumping method Tongqiao group and the comparison group, nourishing Yin, blood group For comparison , the difference is significant, and the P values ​​are P<0.05 , P<0.05 , P<0.01 . Conclusion : Each drug group can improve the memory of mice to varying degrees , and the Tongfu group has the most significant effect.
Key words Chinese medicine, memory impairment, experimental study
Alzheimer's disease, refers to a comprehensive obstacles with significant disturbance of consciousness of the advanced features of the cerebral cortex acquired, including memory power, sensory and motor function, activities of daily living, play social functions, control the language barrier exchanges and emotional response, usually in the form Progressive development. Among them, memory impairment is the most important symptom. According to the pathogenesis of Alzheimer's, the author uses a different rule is, scopolamine hydrobromide in mice caused by memory impairment model acquired a comparative study aimed at screening may be effective in the treatment and medication for the treatment of senile dementia.
1 material method
1.1 Grouping and experimental methods Kunming mice , male , weighing 25 ± 2g, were provided by the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine. Coarse sieve avoidance method, where the latency within 15 seconds, passing, according to results randomly divided into normal, model, Yixin gas, through minded, Yin, blood, nimodipine 7 group. The mice were allowed to stand in a quiet, light-filled environment at room temperature of 20 to 25 ° C for 3 to 5 days , and administration was started. The dose was calculated according to the body surface area equivalent dose folding algorithm , and the dose corresponding to the adult was administered for 10 days ( both 0.3 ml / 10 g · kg - 1 body weight ), and the normal group and the model group were given the same amount of physiological saline. One hour after the last administration , except for the normal group , the other groups were given scopolamine hydrobromide 3 mg/kg, and the neck and back were injected subcutaneously to cause a model of acquired memory impairment. After 10 minutes, avoid darkness and platform training. Determination of short term memory underwent four hours, once retest 24 hours later. Each set of tests is performed at the same time.
1.2 Behavioral test method Ski jumping method : Put the mouse into the reaction box to adapt to the environment for 3 minutes , and immediately pass 36V AC .
After being shocked, the animal jumped back to the platform to avoid noxious stimuli. The time required for animals to escape to the platform is the incubation period. Most animals can jump to the copper grid again or again , and jump back to the platform after being shocked. Training for 5 minutes, record the number and the number of errors per mouse subjected to electric shocks as a training and test scores. Avoid the dark method : Put the mouse face back into the hole in the bright room , and start the timer. According to the habit of avoiding darkness , the animal passed through the hole into the dark room and was shocked by a 40V electric shock to escape to the bright room . The time required for the mouse to escape to the bright room was the incubation period. Most mice can enter the darkroom again or more , and escape to the bright room after being shocked. Training five minutes the number of shocks per mouse by recording the number of errors and, as a training and test scores.
1.3 Statistical processing Group average comparison , analysis of variance , q test. Significant difference P < 0.05 .
2 experimental results
Showed: After 4 hours, short-term memory to do with avoidance method for the determination method and jumping, and the model group jumping avoidance reaction times than normal error significantly increased, five minutes in each treatment group than in model group number of errors is significantly reduced. Compared with the western medicine control group , the difference between the Tongqiao group and the western medicine control group was significant (P<0.05) . The difference between the Tongyu group and the Ziyin and Huoxue groups was significant (the former P<0.05, the latter P<0). 01) . After 24 hours , there was no significant difference in the scores at all levels and the error rate was reduced , which may be related to the metabolic cycle of scopolamine .
3 discussion
Alzheimer's disease is the name of modern medicine, and it has no similar name in the motherland medicine. It is generally believed that the symptoms of the disease are scattered in "Shen", "Forgetfulness", "Depression", and "Crazy". The author believes that "medical medicinal gold and miscellaneous diseases and heart disease" proposed "sacred disease" including various major clinical manifestations of senile dementia . Therefore , the disease should be classified into the category of "sickness" of traditional Chinese medicine. Looking at the works of traditional Chinese medicine in the past , the main organs of the disease are the heart, spleen, liver, kidney and brain. On the cause, I believe, senile dementia syndromes based virtual reality, which includes the virtual heart and kidney essence Qi true, mark it as a real and folders phlegm, stasis. Such as "classification of the ruling" 曰 : "The wife of the lady is in the heart , the essence of the heart depends on the kidney. And the brain is the house of the gods , the essence of the sea , the real memory is also based." "The forgotten forgetter , must pay Its heart and kidney , so that the god of the heart is clear to the kidney , the essence of the kidney rises to the brain. The essence can be angry , the gas can be alive , the god is clear , and the self forgets the loss. "But because of the forgetfulness , ... or sipping , or fascinated... If the blood is inside, forget it is mad." For the cause and disease , the author has developed a heart, nourishing the kidney, and turning Tongqiao sputum, blood circulation is kind of prescription rule, the use of scopolamine hydrobromide in mice caused by acquired impairment of memory model, experimental research carried out to improve the behavior, in order to explore, screening more effective treatment options. The experimental results show that the above four methods have significant improvement in behavior compared with the model group. Among them, the Tongbi group was significantly different from the western medicine control group and the Huoxue and Ziyin groups , indicating that the effect of the overnight method to improve memory is particularly prominent. Looking at the "ancient and modern books Integration · Medical Department recorded various diseases · · palpitation palpitation, forgetfulness door" of the 125 puzzle prescription, with 86% of prescription iris, Polygala, Poria as Tongqiao medicine. The error rate of retesting after 24 hours is extremely low . It may be due to the short half-life of scopolamine hydrobromide , which is only 15 to 40 minutes . Most of the intramuscular injection is excreted within 12 hours . Therefore , the model of memory impairment caused by scopolamine hydrobromide is temporary. The above experiments prove that the motherland medicine has great potential to be exploited in the prevention of senile dementia as a worldwide medical problem. At the same time , we should also see that this experiment is only a rough screening method for the treatment of memory disorders , but this method is simple and easy to use as a screening drug. Of promising drug alternative and more stable, and neurochemical changes similar to Alzheimer's disease in animal models, the mechanism for further discussion and study.
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Chromium Picolinate,Tianeptine,6-Paradol,Aminobutyric acid,acetylcysteine,L-Carnosine
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